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Image Search Results
Journal: Science signaling
Article Title: Targeting the ion channel TRPM7 promotes the thymic development of regulatory T cells by promoting IL-2 signaling
doi: 10.1126/scisignal.abb0619
Figure Lengend Snippet: (A) The experimental scheme used for the suppression assays. (B) Analysis of CFSE-labeled WT T cell proliferation after 3 days of culture in the presence of Treg cells isolated from WT (blue) and KO (red) mice. The ratios indicate the relative numbers of CD4+CD25− Teff cells to CD4+CD25+ Treg cells plated on day 1. Histograms show the dilution of CFSE in Teff cells as measured by flow cytometry. The percentage of Teff cells showing dilution of the CFSE dye reflects the proportion of Teff cells that underwent cell division. Data are representative of four experiments. (C) Treg cells from the thymi and spleens of WT (blue) and KO (red) mice were identified by flow cytometry (Left: bivariant gating for CD4 and Foxp3) and further analyzed for the expression of the Treg cell functional markers CD39, CD73, and CTLA4 (histograms). (D) Percentages of positive cells (left) and MFI values (right) for CD39, CD73, and CTLA4 in thymic and splenic CD4+Foxp3+ Tregs from WT and KO mice. The data were collected from two independent experiments with a total sample size of 4 mice. The P value was calculated by one-tailed unpaired t-test. (E) Cell surface expression of neuropilin-1 (Nrp-1) on thymic and splenic Treg cells (CD4+Foxp3+) from WT (blue) and KO (red) mice. (F) Bar graphs showing the percentage of positive cells (left) and MFI values (right) for Nrp-1 in thymic and splenic T cells from WT and KO mice. The data were collected from two independent experiments with a total sample size of four mice. The P value was calculated by one-tailed unpaired t-test.
Article Snippet: The following antibodies were used: anti-mouse neuropilin-1-PE (R&D Systems, #FAB5994P), rat anti-mouse CD25 PE-Cy7 (BD Pharmingen, #552880), anti-mouse CD39 PE-Cyanine7 (eBioscience, Inc. #25-0391), anti-mouse CD73 PE (eBioscience, Inc. #12-0731), rat anti-mouse CD4 FITC (BD Biosciences #553046), purified hamster anti-mouse CD28 (BD Biosciences #553294), anti-mouse/rat Foxp3 APC clone FJK-16s (eBioscience), anti-Foxp3 (eBioscience, #17-5773), purified hamster anti-mouse CD3ε chain, clone 145-2C11 (BD Biosciences #553058),
Techniques: Labeling, Isolation, Flow Cytometry, Expressing, Functional Assay, One-tailed Test
Journal: Cell Reports Medicine
Article Title: Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma
doi: 10.1016/j.xcrm.2021.100399
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Infection, Transfection, Cell Isolation, Selection, Staining, Sequencing, Transgenic Assay, Expressing, Software